Stops along this trip:
| Time | Stop Number | Stop Name | |
|---|---|---|---|
| 1 | 04:55 | 4051 | Bridge / Northfield |
| 2 | 04:56 | 4038 | Bridge / Chesapeake (North) |
| 3 | 04:57 | 4036 | Bridge / Chesapeake (South) |
| 4 | 04:57 | 4035 | Bridge / Eastbridge |
| 5 | 04:59 | 3784 | Bridge / Lexington |
| 6 | 04:59 | 3679 | Bridge / Dansbury |
| 7 | 05:00 | 3680 | Bridge / Crimson |
| 8 | 05:01 | 4032 | Bridge / University Ave. |
| 9 | 05:01 | 4030 | Bridge / Bechtel Park |
| 10 | 05:02 | 1941 | Bridge / Bridle |
| 11 | 05:03 | 1942 | Bridge / Woolwich |
| 12 | 05:03 | 1943 | Lancaster / Bridge |
| 13 | 05:04 | 1944 | Lancaster / General |
| 14 | 05:04 | 1945 | Lancaster / Lang |
| 15 | 05:05 | 2440 | Lancaster / Hamel |
| 16 | 05:06 | 2441 | Lancaster / Union |
| 17 | 05:07 | 2442 | Lancaster / Ash |
| 18 | 05:08 | 2444 | Lancaster / Guelph |
| 19 | 05:08 | 2445 | Lancaster / Hill |
| 20 | 05:09 | 2447 | Wellington / Major |
| 21 | 05:10 | 2448 | Wellington / St Leger |
| 22 | 05:11 | 2449 | Wellington / Margaret |
| 23 | 05:13 | 3744 | Welington / Weber |
| 24 | 05:14 | 2539 | Victoria / Kitchener Train Station |
| 25 | 05:15 | 1474 | Central Station |
| 26 | 05:16 | 1273 | Charles / Water |
| 27 | 05:18 | 1385 | Queen Station (Charles / Benton) |
| 28 | 05:19 | 2924 | Kitchener Market Station |
| 29 | 05:19 | 2925 | Charles / Cameron Heights Collegiate Institute |
| 30 | 05:20 | 2927 | Stirling / Kaufman Park |
| 31 | 05:21 | 5000 | Courtland / Vernon (Temporary Stop) |
| 32 | 05:21 | 2718 | Courtland / Palmer |
| 33 | 05:22 | 2719 | Courtland / Kent |
| 34 | 05:22 | 2721 | Courtland / Grenville |
| 35 | 05:23 | 2722 | Courtland / Sydney |
| 36 | 05:24 | 2724 | Courtland / Carwood |
| 37 | 05:25 | 2725 | Courtland / Overland |
| 38 | 05:26 | 2115 | Courtland / Hayward |
| 39 | 05:27 | 1463 | Block Line Station |
| 40 | 05:29 | 2730 | Vanier / Vanier Park |
| 41 | 05:30 | 2731 | Vanier / Siebert |
| 42 | 05:31 | 2732 | Traynor / Vanier |
| 43 | 05:31 | 2733 | Traynor / Belwood |
| 44 | 05:32 | 2734 | Traynor / Balfour |
| 45 | 05:33 | 2594 | Wilson / Traynor |
| 46 | 05:33 | 2595 | Wilson / Balfour |
| 47 | 05:35 | 3495 | Fairway Station |
Effective: April 27, 2026 - June 19, 2026