Stops along this trip:
| Time | Stop Number | Stop Name | |
|---|---|---|---|
| 1 | 22:15 | 3495 | Fairway Station |
| 2 | 22:16 | 2497 | Wilson / Balfour |
| 3 | 22:16 | 2506 | Wilson / Traynor |
| 4 | 22:17 | 2736 | Traynor / Balfour |
| 5 | 22:18 | 2737 | Tarynor / Belwood |
| 6 | 22:19 | 2738 | Traynor / Vanier |
| 7 | 22:19 | 2740 | Vanier / Siebert |
| 8 | 22:20 | 1905 | Vanier / Shelley |
| 9 | 22:21 | 1906 | Shelley / Courtland |
| 10 | 22:22 | 2349 | Block Line Station |
| 11 | 22:24 | 2296 | Courtland / Walton |
| 12 | 22:25 | 2745 | Courtland / Carwood |
| 13 | 22:27 | 2746 | Courtland / Sydney |
| 14 | 22:27 | 2748 | Courtland / Borden |
| 15 | 22:28 | 2749 | Courtland / Palmer |
| 16 | 22:29 | 2915 | Stirling / Courtland |
| 17 | 22:29 | 2916 | Stirling / Kaufman Park |
| 18 | 22:31 | 2919 | Charles / Cameron Heights Collegiate Institute |
| 19 | 22:31 | 2920 | Kitchener Market Station |
| 20 | 22:32 | 1376 | Queen Station (Charles / Benton) |
| 21 | 22:33 | 1313 | Victoria Park Station |
| 22 | 22:34 | 1292 | Charles / Water |
| 23 | 22:35 | 3226 | Central Station |
| 24 | 22:36 | 2541 | Victoria / Duke |
| 25 | 22:38 | 5049 | Wellington / Weber (Temporary Stop) |
| 26 | 22:39 | 2414 | Wellington / Margaret |
| 27 | 22:40 | 2415 | Wellington / St Leger |
| 28 | 22:40 | 2416 | Wellington / Major |
| 29 | 22:41 | 2418 | Lancaster / Hill |
| 30 | 22:42 | 2419 | Lancaster / Guelph |
| 31 | 22:43 | 2421 | Lancaster / Ash |
| 32 | 22:43 | 2422 | Lancaster / Union |
| 33 | 22:44 | 2423 | Lancaster / Hamel |
| 34 | 22:45 | 2020 | Lancaster / Bridgeport Rd. |
| 35 | 22:45 | 2021 | Lancaster / General |
| 36 | 22:46 | 2424 | Lancaster / Bridge |
| 37 | 22:47 | 2022 | Bridge / Woolwich |
| 38 | 22:48 | 2023 | Bridge / Bridle |
| 39 | 22:49 | 4031 | Bridge / Path To Auburn |
| 40 | 22:49 | 4033 | Bridge / University Ave. |
| 41 | 22:50 | 3673 | Bridge / Crimson |
| 42 | 22:51 | 4034 | Bridge / Dansbury |
| 43 | 22:51 | 3789 | Bridge / Lexington |
| 44 | 22:53 | 1925 | Bridge / Eastbridge |
| 45 | 22:53 | 4037 | Bridge / Chesapeake (South) |
| 46 | 22:54 | 4016 | Bridge / Chesapeake (North) |
| 47 | 22:55 | 3756 | Northfield / Bridge |
| 48 | 22:56 | 4009 | 120 Northfield Dr. E. |
| 49 | 22:57 | 2088 | Northfield / Wissler |
| 50 | 22:57 | 4043 | Northfield / Davenport |
| 51 | 22:58 | 1212 | Kraus / Northfield |
| 52 | 23:00 | 1126 | Conestoga Station |
Effective: April 26, 2026 - April 26, 2026