Stops along this trip:
| Time | Stop Number | Stop Name | |
|---|---|---|---|
| 1 | 22:54 | 1000 | Frederick Station (Frederick / King) |
| 2 | 22:55 | 2319 | Weber / Queen |
| 3 | 22:55 | 2320 | Queen / Kitchener Central Library |
| 4 | 22:56 | 2654 | Margaret / Centre In The Square |
| 5 | 22:57 | 2690 | Margaret / Victoria |
| 6 | 22:58 | 2657 | Margaret / Wellington |
| 7 | 22:59 | 2658 | Margaret / Wilhelm |
| 8 | 22:59 | 2659 | Margaret / Guelph |
| 9 | 23:00 | 2491 | Margaret / Breithaupt Centre |
| 10 | 23:01 | 2492 | Union / Margaret |
| 11 | 23:01 | 2493 | Union / Ellis |
| 12 | 23:02 | 2660 | Union / Weber |
| 13 | 23:03 | 5136 | Dover / Rodney (Temporary Stop) |
| 14 | 23:04 | 5142 | Allen / Willow (Temporary Stop) |
| 15 | 23:06 | 2538 | King / Union |
| 16 | 23:06 | 1272 | Grand River Hospital Station |
| 17 | 23:07 | 3242 | Park / Wood |
| 18 | 23:07 | 3243 | Park / Green |
| 19 | 23:08 | 3244 | Glasgow / York |
| 20 | 23:08 | 3245 | Glasgow / Eden |
| 21 | 23:09 | 3246 | Glasgow / Belmont |
| 22 | 23:09 | 3248 | Glasgow / Dunbar |
| 23 | 23:10 | 3249 | Glasgow / Westmount |
| 24 | 23:11 | 3250 | Glasgow / Aberdeen |
| 25 | 23:13 | 5006 | Westwood / Knell (Temporary Stop) |
| 26 | 23:13 | 3256 | Westwood / Gallarno |
| 27 | 23:15 | 3258 | Westwood / Glasgow |
| 28 | 23:15 | 3259 | Fischer-Hallman / Glasgow |
| 29 | 23:16 | 3260 | University Ave. / Fischer-Hallman |
| 30 | 23:17 | 3879 | University Ave. / Westvale |
| 31 | 23:18 | 3880 | University Ave. / Portsmouth |
| 32 | 23:19 | 3662 | University Ave. / Baker |
| 33 | 23:20 | 4003 | The Boardwalk Station |
Effective: June 06, 2026 - June 20, 2026