Effective: April 22, 2024 - June 21, 2024
Stops along this trip:
| Time | Stop Number | Stop Name | |
|---|---|---|---|
| 1 | 05:33 | 1376 | Queen Station (Charles / Benton) |
| 2 | 05:34 | 1313 | Victoria Park Station |
| 3 | 05:35 | 1292 | Charles / Water |
| 4 | 05:36 | 3226 | Central Station |
| 5 | 05:37 | 2541 | Victoria / Duke |
| 6 | 05:39 | 5049 | Wellington / Weber (Temporary Stop) |
| 7 | 05:39 | 2413 | Wellington / Ahrens |
| 8 | 05:40 | 2414 | Wellington / Margaret |
| 9 | 05:41 | 2415 | Wellington / St Leger |
| 10 | 05:41 | 2416 | Wellington / Major |
| 11 | 05:42 | 2417 | Lancaster / Louisa |
| 12 | 05:42 | 2418 | Lancaster / Hill |
| 13 | 05:43 | 2419 | Lancaster / Guelph |
| 14 | 05:43 | 2420 | Lancaster / Arnold |
| 15 | 05:44 | 2421 | Lancaster / Ash |
| 16 | 05:44 | 2422 | Lancaster / Union |
| 17 | 05:45 | 3657 | Lancaster / Highway 85 |
| 18 | 05:45 | 2423 | Lancaster / Hamel |
| 19 | 05:46 | 2020 | Lancaster / Bridgeport Rd. |
| 20 | 05:46 | 2021 | Lancaster / General |
| 21 | 05:47 | 2424 | Lancaster / Bridge |
| 22 | 05:48 | 2022 | Bridge / Woolwich |
| 23 | 05:49 | 2023 | Bridge / Bridle |
| 24 | 05:49 | 4031 | Bridge / Path To Auburn |
| 25 | 05:50 | 4033 | Bridge / University Ave. |
| 26 | 05:50 | 3672 | Bridge / University Ave. |
| 27 | 05:51 | 3673 | Bridge / Crimson |
| 28 | 05:52 | 4034 | Bridge / Dansbury |
| 29 | 05:52 | 3789 | Bridge / Lexington |
| 30 | 05:53 | 3790 | Bridge / Whitmore |
| 31 | 05:54 | 1925 | Bridge / Eastbridge |
| 32 | 05:54 | 4037 | Bridge / Chesapeake (South) |
| 33 | 05:55 | 4028 | Bridge / Path To Brigantine |
| 34 | 05:56 | 4016 | Bridge / Chesapeake (North) |
| 35 | 05:56 | 4050 | Bridge / Labrador |
| 36 | 05:58 | 4009 | 120 Northfield Dr. E. |
| 37 | 05:58 | 2088 | Northfield / Wissler |
| 38 | 05:59 | 4043 | Northfield / Davenport |
| 39 | 05:59 | 1212 | Kraus / Northfield |
| 40 | 06:01 | 1126 | Conestoga Station |